Due to large demand in biopharmaceuticals in the coming future researchers are directing their attention to large-scale studies to elucidate biomolecule functions. Such studies require automated methods to overcome bottlenecks associated with purification. For this purpose BioOnco is developing Beads/Rods, a simple and rapid system for high-throughput, small-scale purification of bio molecules in an automated format. The surface of the beads can be derivatized with certain chemistries that will allow to capture biomolecues adhere to the surface in order to perform purification. Samples containing the molecule of interest are incubated with beads that are dramatized with an antibody or other binding partner. Most commonly, ligands are immobilized or “coupled” directly to solid support material by formation of covalent chemical bonds between particular functional groups on the ligand (e.g., primary amines, sulfhydryls, carboxylic acids, aldehydes) and reactive groups on the support . Our ‘High-throughput’ platform will systematically study a series of biophysical parameters such as maximum surface coverage ,particle size and morphology of the beads in order to optimize the separation very well.
